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Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion.
Hu L, Qin X, Huang Y, Cao W, Wang C, Wang Y, Ling X, Chen H, Wu D, Lin Y, Liu T
ACS Synth Biol. 2020 Oct 16;9(10):2723-2736. doi: 10.1021/acssynbio.0c00257. Epub 2020 Sep 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
164080pCMV-MtPylRS (human-opti)To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion
164081pCMV-MfPylRS (human-opti)To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion
164195pCMV-MtAcKRS (human-opti)To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
164196pCMV-MfBulKRS (human-opti)To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

Antibodies from Article