PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||164195||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5988
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1434
MutationD76G; L325M; L329I; Y330F; L333A; C372F
- Promoter CMV
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGHR (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
the insert gene-MtPylRS was retrieved from the uniprot database, where its ID is Q1L6A3. The genetic codon of the insert gene has been human optimized
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-MtAcKRS (human-opti) was a gift from Tao Liu (Addgene plasmid # 164195 ; http://n2t.net/addgene:164195 ; RRID:Addgene_164195)
For your References section:Thermophilic Pyrrolysyl-tRNA Synthetase Mutants for Enhanced Mammalian Genetic Code Expansion. Hu L, Qin X, Huang Y, Cao W, Wang C, Wang Y, Ling X, Chen H, Wu D, Lin Y, Liu T. ACS Synth Biol. 2020 Oct 16;9(10):2723-2736. doi: 10.1021/acssynbio.0c00257. Epub 2020 Sep 23. 10.1021/acssynbio.0c00257 PubMed 32931698