ER-directed TREX1 limits cGAS activation at micronuclei.
Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 10.1016/j.molcel.2020.12.037. PubMed Article
Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 10.1016/j.molcel.2020.12.037. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164225 | pLENTI-CMV-GFP-TREX1 D18N BLAST | lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient) |
| 164228 | pLENTI-CMV-GFP-TREX1 BLAST | lentiviral plasmid for GFP-hTREX1 |
| 164229 | pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST | lentiviral plasmid for GFP-hTREX1 delta C (aa1-235) |
| 164231 | pQCXIP-GFP-RPA70 | retroviral plasmid for GFP-RPA70 |
| 164232 | pQCXIZ-turq-3xFLAG-TREX1 | retroviral plasmid for turq-3xFLAG-TREX1 |
| 164233 | pQCXIZ-turq-3xFLAG-TREX1 D18N | retroviral plasmid for turq-3xFLAG-TREX1 D18N (catalytically-deficient) |
| 164234 | pLENTI CMV GFP TREX1 R128A R174A BLAST | lentiviral plasmid for GFP-TREX1 R128A R174A |
| 164235 | pLENTI CMV GFP-TREX1 delta N (aa236-314) BLAST | lentiviral plasmid for GFP-TREX1 delta N (aa236-314) |
| 164236 | pLENTI CMV GFP-TREX1 V235fs BLAST | lentiviral plasmid for GFP-TREX1 V235fs |
| 164237 | pLENTI CMV GFP-TREX1 D272fs BLAST | lentiviral plasmid for GFP-TREX1 D272fs |
| 164238 | pLENTI CMV GFP-TREX1 L287fs BLAST | lentiviral plasmid for GFP-TREX1 L287fs |
| 164239 | pLENTI CMV GFP-TREX1 P290L BLAST | lentiviral plasmid for GFP-TREX1 P290L |
| 164240 | pLENTI CMV GFP-TREX1 Y305C BLAST | lentiviral plasmid for GFP-TREX1 Y305C |
| 164241 | pLENTI CMV GFP-TREX1 G306A BLAST | lentiviral plasmid for GFP-TREX1 G306A |
| 164242 | pLENTI CMV GFP-TREX1 309* BLAST | lentiviral plasmid for GFP-TREX1 309* |
| 164243 | pQCXIZ 3xFLAG-TREX1 | retroviral plasmid for 3xFLAG-TREX1 |
| 164244 | pQCXIB-H2B-mCherry | retroviral plasmid for H2B-mCherry |
| 164245 | pQCXIP GFP-TREX1 | retroviral plasmid for GFP-TREX1 |
| 164248 | pQCXIP-GFP-BAF | retroviral plasmid for GFP-BAF |
| 164249 | pQCXIP-GFP-LmnB1 | retroviral plasmid for GFP-LmnB1 |
| 164250 | pU6-TREX1 g1 Cas9-T2A-mCherry | Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |
| 164251 | pU6-TREX1 g2 Cas9-T2A-mCherry | Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |
| 164252 | pU6-TREX1 g3 Cas9-T2A-mCherry | Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |
| 164253 | pU6-cGAS g2 Cas9-T2A-mCherry | Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |
| 164254 | pU6-cGAS g3 Cas9-T2A-mCherry | Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |
| 164255 | pHP138 Myc-TRF2-deltaB deltaM | for generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmid |
| 164256 | pU6-p53 gRNA-Cas9-T2A-mCherry | Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells |