X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes in the immune system.
LaFleur MW, Lemmen AM, Streeter ISL, Nguyen TH, Milling LE, Derosia NM, Hoffman ZM, Gillis JE, Tjokrosurjo Q, Markson SC, Huang AY, Anekal PV, Montero Llopis P, Haining WN, Doench JG, Sharpe AH
Nat Immunol. 2023 Nov 27. doi: 10.1038/s41590-023-01689-6. PubMed Article
LaFleur MW, Lemmen AM, Streeter ISL, Nguyen TH, Milling LE, Derosia NM, Hoffman ZM, Gillis JE, Tjokrosurjo Q, Markson SC, Huang AY, Anekal PV, Montero Llopis P, Haining WN, Doench JG, Sharpe AH
Nat Immunol. 2023 Nov 27. doi: 10.1038/s41590-023-01689-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 164265 | pXPR_070 | Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction. |
| 164555 | pXPR_068 | Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction. |
| 164558 | pXPR_071 | Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction. |
| 164559 | pXPR_219 | Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction. |
| 164560 | pXPR_220 | Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction. |