Addgene: CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes.

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Nicholas Ingolia
Muller et al

CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes.
Muller R, Meacham ZA, Ferguson L, Ingolia NT
Science. 2020 Dec 11;370(6522). pii: 370/6522/eabb9662. doi: 10.1126/science.abb9662. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
164912	pNTI732 pRPR1(TetO)-HTS1-sgRNA	For yeast genomic integration of sgRNA against HTS1
164913	pNTI733 pRPR1(TetO)-RPC31-sgRNA	For yeast genomic integration of sgRNA against RPC31
164915	pNTI743 dual-barcoded gRNA parent vector	CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries
164916	pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)	Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR
164917	pNTI757 P(PGK1)-citrine P(HIS4)-mCherry	Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer
164918	pNTI763 p(Z)-mCherry + UBC9 sgRNA	CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Antibodies from Article