High-throughput cloning and expression of integral membrane proteins in Escherichia coli.
Bruni R, Kloss B
Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34. doi: 10.1002/0471140864.ps2906s74. PubMed Article
Bruni R, Kloss B
Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34. doi: 10.1002/0471140864.ps2906s74. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165477 | pC23-LIC-His-FLAG | pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His |
| 165478 | pN23-LIC-His-FLAG | pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His |
| 165479 | pC28-LIC-sfGFP-His | pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag |
| 165480 | pRB30 | pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal Flag and His |
| 165481 | pRB30-GFP | pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His |
| 165482 | pRB33 | pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His |
| 165483 | pRB33-GFP | pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal uv-GFP, Flag and His |