Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays.
Mehalko J, Drew M, Snead K, Denson JP, Wall V, Taylor T, Sadtler K, Messing S, Gillette W, Esposito D
Protein Expr Purif. 2021 Mar;179:105802. doi: 10.1016/j.pep.2020.105802. Epub 2020 Nov 25. PubMed Article
Mehalko J, Drew M, Snead K, Denson JP, Wall V, Taylor T, Sadtler K, Messing S, Gillette W, Esposito D
Protein Expr Purif. 2021 Mar;179:105802. doi: 10.1016/j.pep.2020.105802. Epub 2020 Nov 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166018 | R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 | mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) |
| 166019 | R619-M68-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP | mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology |
| 166020 | R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP | mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology |
| 170189 | R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant |
| 170190 | R620-M04-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP N501Y | ammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant |
| 170191 | R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant |
| 170192 | R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant |
| 170193 | R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant |
| 170194 | R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant |
| 170202 | R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant |
| 170203 | R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y | Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant |