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Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.
Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA
Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 10.1242/bio.055343.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
169895pNanoMinimal cloning vector
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169897pEPIC1.1Empty constitutive expression vector with piggyBac, IRES, and mClover3
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169898pPID2Minimal empty vector with piggyBac, insulators, and a DTS
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169899pPIDCBEmpty dox inducible vector with piggyBac, insulators, and a DTS
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169900pPIDCB2Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.
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169901pPIDNBEmpty dox inducible vector with piggyBac, insulators, and a DTS
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169902pPIDNB2Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.
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Antibodies from Article