Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath.
Ren X, Wang M, Li B, Jamieson K, Zheng L, Jones IR, Li B, Takagi MA, Lee J, Maliskova L, Tam TW, Yu M, Hu R, Lee L, Abnousi A, Li G, Li Y, Hu M, Ren B, Wang W, Shen Y
Sci Adv. 2021 Sep 17;7(38):eabi4360. doi: 10.1126/sciadv.abi4360. Epub 2021 Sep 15. PubMed Article
Ren X, Wang M, Li B, Jamieson K, Zheng L, Jones IR, Li B, Takagi MA, Lee J, Maliskova L, Tam TW, Yu M, Hu R, Lee L, Abnousi A, Li G, Li Y, Hu M, Ren B, Wang W, Shen Y
Sci Adv. 2021 Sep 17;7(38):eabi4360. doi: 10.1126/sciadv.abi4360. Epub 2021 Sep 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167186 | lentiCRISPR-v2-Blast-Puro | Lentiviral expression of sgRNA with blasticidin and puromycin resistance genes |
| 167188 | lentiCRISPR-v2-HygR-EGFP | Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP |
| 167189 | lentiCRISPR-v2-HygR-mCherry | Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry |