lentiCRISPR-v2-HygR-mCherry
(Plasmid
#167189)
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PurposeLentiviral expression of sgRNA with hygromycin resistance gene and mCherry
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 167189 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonelentiCRISPR v2 (Addgene #52961)
- Backbone size w/o insert (bp) 14873
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Modifications to backboneReplaced the Cas9 sequence in lentiCRISPR v2 plasmid (Addgene, 52961) with hygromycin resistance gene and mCherry
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHygromycin B phosphotransferase and mCherry
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Insert Size (bp)1812
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiCRISPR-v2-HygR-mCherry was a gift from Yin Shen (Addgene plasmid # 167189 ; http://n2t.net/addgene:167189 ; RRID:Addgene_167189) -
For your References section:
Parallel characterization of cis-regulatory elements for multiple genes using CRISPRpath. Ren X, Wang M, Li B, Jamieson K, Zheng L, Jones IR, Li B, Takagi MA, Lee J, Maliskova L, Tam TW, Yu M, Hu R, Lee L, Abnousi A, Li G, Li Y, Hu M, Ren B, Wang W, Shen Y. Sci Adv. 2021 Sep 17;7(38):eabi4360. doi: 10.1126/sciadv.abi4360. Epub 2021 Sep 15. 10.1126/sciadv.abi4360 PubMed 34524848
Map uploaded by the depositor.
