Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives.
Kozyczkowska A, Najle SR, Ocana-Pallares E, Areste C, Shabardina V, Ara PS, Ruiz-Trillo I, Casacuberta E
Curr Biol. 2021 Sep 27;31(18):4104-4110.e5. doi: 10.1016/j.cub.2021.06.061. Epub 2021 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 104446 | Clim Actin MCH Puro (CAMP) | Expresses mCherry under endogenous actin promoter. It can be used to transfect Corallochytrium limacisporum (Corallochytrea) cells and visualize them by fluorescent microscope. Puromycin resistant. |
| 129556 | Clim-DC2-H2B2-Venus | pClimDC-H2B:mV nuclear marker |
| 129557 | Clim-DC2-NMM-tdtomato | pClimDC-NMM:tdT cell membrane marker |
| 129558 | Clim-DC2-lifeact-mCherry | pClimDCLifeAct:mCh actin filaments marker |
| 129560 | CTMP | cytoplasmic marker |