A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish.
Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding C, Lemke AP, Zhang G, Tobin DM, Deng Q
J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 10.1242/jcs.258574. Epub 2021 Apr 22. PubMed Article
Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding C, Lemke AP, Zhang G, Tobin DM, Deng Q
J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 10.1242/jcs.258574. Epub 2021 Apr 22. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168239 | Tol2-LyzC-Cas9-pA-Cry-GFP | "neutrophil specific cas9 expression in zebrafish with green lens" |
| 168240 | Tol2-LyzC-GFP-U6ac-ctrl-guides | "neutrophil specific GFP with ubiquitous ctrl sgRNAs" |
| 168241 | Tol2-LyzC-GFP-U6ac-rac2-guides | "neutrophil specific GFP with ubiquitous rac2 sgRNAs" |
| 168242 | Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides | "Rescue Rac expression in neutrophil specific knockout" |
| 168243 | Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides | "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout" |
| 168244 | Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides | "Rescue Rac-CA expression in neutrophil specific knockout" |
| 168247 | Tol2-LyzC-RacFRET-U6ac-ctrl guides | "label active Rac; control for neutrophil-specific knockout" |
| 168248 | Tol2-LyzC-RacFRET-U6ac-rac2 guides | "label active Rac under Rac2 neutrophil-specific knockout background" |
| 168250 | Tol2-LyzC-GFP-U6ac-cdk2-guides | "neutrophil specific GFP with ubiquitous cdk2 sgRNAs" |
| 168251 | Tol2-LyzC-PBD-GFP-U6ac-rac2 guides | "label active Rac under Rac2 neutrophil-specific knockout background" |
| 168252 | Tol2-LyzC-PBD-GFP-U6ac-ctrl guides | "label active Rac; control for neutrophil-specific knockout" |
| 168253 | Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides | "label stable F-actin; control for neutrophil-specific knockout" |
| 168254 | Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides | "label stable F-actin under Rac2 neutrophil-specific knockout background" |