Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp3 papain-like protease.
Lim CT, Tan KW, Wu M, Ulferts R, Armstrong LA, Ozono E, Drury LS, Milligan JC, Zeisner TU, Zeng J, Weissmann F, Canal B, Bineva-Todd G, Howell M, O'Reilly N, Beale R, Kulathu Y, Labib K, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2517-2531. doi: 10.1042/BCJ20210244. PubMed Article
Lim CT, Tan KW, Wu M, Ulferts R, Armstrong LA, Ozono E, Drury LS, Milligan JC, Zeisner TU, Zeng J, Weissmann F, Canal B, Bineva-Todd G, Howell M, O'Reilly N, Beale R, Kulathu Y, Labib K, Diffley JFX
Biochem J. 2021 Jul 16;478(13):2517-2531. doi: 10.1042/BCJ20210244. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169192 | pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2) | To express SARS-CoV-2 Papain-like protease in E. coli |
| 169193 | pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2) | To express SARS-CoV-2 Papain-like protease in E. coli |
| 169194 | pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2) | Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells |
| 169195 | pGEX4T1_GST-PLproCS-MBP | To express a PLpro substrate (GST-PLproCS-MBP) in E. coli |