Michael Ward Lab:
Characterization of iPSC lines for the iPSC Neurodegenerative Disease Initiative (iNDI)
Unpublished
Unpublished
Please see Addgene's curated iPSC Neurodegenerative Disease Initiative (iNDI) Collection page for additional information about the collection.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182306 | PB-TO-NFIA.1-SOX9 (bi-directional promoter) | Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression |
| 182307 | PB-TO-ASCL1-DLX2 | Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression |
| 182308 | PB-TO-hNGN2-BSD-mApple | Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple |
| 182309 | PB-TO-MYOD1-shOct4 | Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression |
| 182310 | PB-TO-NFIA.1-SOX9 | Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression |
| 182311 | PB-TO-NGN2-EMX1 BSD-mApple | Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple |
| 182312 | PB-TO-NGN2-EMX1 puro-BFP | Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, puromycin selection, BFP |
| 182313 | PB-TO-hNIL BSD-mApple | Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple |
| 198397 | PB-TO-oNGN2 | Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool. |
| 204733 | iC13 PB-TO-oNgn2-BFP-puro | Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase. |
| 204734 | iF59 PB-TO-oNgn2-puro | Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase. |