A TXTL-Based Assay to Rapidly Identify PAMs for CRISPR-Cas Systems with Multi-Protein Effector Complexes.

Wimmer F, Englert F, Beisel CL
Methods Mol Biol. 2022;2433:391-411. doi: 10.1007/978-1-0716-1998-8_24. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
170088	pEc-crRNA1	encodes E. coli type I-E single spacer array
170089	pEc-crRNA2	encodes E. coli type I-E single spacer array
170090	pEcCas5	expresses E. coli type I-E Cas5
170091	pEcCas6	expresses E. coli type I-E Cas6
170092	pEcCas7	expresses E. coli type I-E Cas7
170093	pEcCas8	expresses E. coli type I-E Cas8
170094	pEcCse2	expresses E. coli type I-E Cse2
170095	pGFP-ATAAC	deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter
170096	pGFP-CAAAG	deGFP reporter plasmid with CAAAG PAM upstream of p70a promoter
170097	pGFP-CAATG	deGFP reporter plasmid with CAATG PAM upstream of p70a promoter
170098	pGFP-GTAAT	deGFP reporter plasmid with GTAAT PAM upstream of p70a promoter
170099	pGFP-GTATT	deGFP reporter plasmid with GTATT PAM upstream of p70a promoter
170100	pGFP-PacI	deGFP reporter plasmid with unique PacI restriction enzyme recognition site
170101	pT7RNAP	expresses T7 RNAP