Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits.
Chen J, Poskanzer KE, Freeman MR, Monk KR
Nat Neurosci. 2020 Oct;23(10):1297-1306. doi: 10.1038/s41593-020-0703-x. Epub 2020 Sep 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
170205p5E-slc1a3bGateway 5’ entry vector with the slc1a3b promoter
170206pME-mCD8mCherryGateway middle entry vector that encodes mCD8mCherry
170207slc1a3b:mCD8mCherryDrives mCD8mCherry expression with the slc1a3b promoter in astrocytes
170208slc1a3b:myrGFP-P2A-H2AmCherryDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus
170209slc1a3b:myrGCaMP6s-P2A-H2AmCherryDrives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

Antibodies from Article