Functional visualization of NK Cell-mediated killing of metastatic single tumor cells.
Ichise H, Tsukamoto S, Hirashima T, Konishi Y, Oki C, Tsukiji S, Iwano S, Miyawaki A, Sumiyama K, Terai K, Matsuda M
Elife. 2022 Feb 3;11. pii: 76269. doi: 10.7554/eLife.76269.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170283 | pPBbsr2-Necl-5-ScNeo | To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein |
| 170284 | pT2Aneo-tdTomato-CAAX | Encoding membrane-anchored tdTomato. |
| 170290 | pT2ADW-lox-mCherry-hyBRET-ERK-NLS | For generating the conditional transgenic mice expressing BRET-FRET hybrid biosensor of ERK. |
| 170293 | LentiCRISPRv2puro-Tyr | A knock-out vector for the mouse tyrosinase. |
| 170294 | LentiCRISPRv2bleo-Necl-5 | A knock-out vector for the mouse Necl-5 |
| 170295 | LentiCRISPRv2bleo-Nectin-2 | A knock-out vector for the mouse Nectin-2 |