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Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion.
Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM
Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 10.1016/j.cell.2021.05.015. Epub 2021 Jun 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
170808pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygRPiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
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170809pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastRLentiviral vector to co-express an spsgRNA with NLS-mNeonGreen
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170810pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastRLentiviral vector to co-express an spsgRNA with NLS-mScarlet-I
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170811pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastRLentiviral vector to co-express an sasgRNA with NLS-mNeonGreen
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170812pW214-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastRLentiviral vector to co-express an sasgRNA with NLS-mScarlet-I
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170813pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastRLentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I
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170814pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastRLentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreen
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170815pW266-lenti-spsgRNA-hsCDH1-sg2-pEF1s-NLS-mScarlet-I-P2A-BlastRLentiviral vector to co-express a human CDH1 spsgRNA (sg2-Cdh1) with NLS-mScarlet-I
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170816pW267-lenti-spsgRNA-hsCDH1-sg1-pEF1s-NLS-mScarlet-I-P2A-BlastRLentiviral vector to co-express a human CDH1 spsgRNA (sg1-Cdh1) with NLS-mScarlet-I
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170817pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastRLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I
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Recombinant Antibodies from Article