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pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR
(Plasmid #170808)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170808 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 170808-D50 50 μg of DNA in Tris buffer $495
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DNA 170808-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSLQ2842 (Addgene #84244)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GID1-VPR-IRES-HygR
  • Species
    A. thaliana (mustard weed), Synthetic; E. coli
  • Insert Size (bp)
    4281
  • Mutation
    Synonymous mutation in PYL1 to remove XhoI site (inherited from pSLQ2842; Addgene #84244)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GAI-tagBFP-Sa_dCas9
  • Species
    A. thaliana (mustard weed), Synthetic; S. aureus
  • Insert Size (bp)
    4275
  • Entrez Gene
    GAI (a.k.a. AT1G14920, F10B6.34, F10B6_34, GAI PROTEIN, GIBBERELLIC ACID INSENSITIVE, RESTORATION ON GROWTH ON AMMONIA 2, RGA2)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR was a gift from Kenneth Yamada (Addgene plasmid # 170808 ; http://n2t.net/addgene:170808 ; RRID:Addgene_170808)

  • For your References section:

    Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Wang S, Matsumoto K, Lish SR, Cartagena-Rivera AX, Yamada KM. Cell. 2021 Jul 8;184(14):3702-3716.e30. doi: 10.1016/j.cell.2021.05.015. Epub 2021 Jun 15. 10.1016/j.cell.2021.05.015 PubMed 34133940
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