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Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines.
Tromp A, Wang H, Hall TE, Mowry B, Giacomotto J
Front Physiol. 2023 Aug 2;14:1221310. doi: 10.3389/fphys.2023.1221310. eCollection 2023. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171792469_pME_iCrepME-Vector with codon improved iCre sequence. Tol2-kit compatible.
171793141_pT3TS_iCrePlasmid for optimised iCre RNA synthesis using T3 promoter.
1717941455_pDEST_miniTol2_R4-R2_MCSpDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.
1717951456_pDEST_miniTol2_R4-R2_Cryst-eGFPpDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)
1717961457_pDEST_miniTol2_R4-R2_Cryst_mCherrypDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)
171797114_UBI_iCre_crystGFPTol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

Antibodies from Article