Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces.

Ye S, Enghiad B, Zhao H, Takano E
J Ind Microbiol Biotechnol. 2020 May;47(4-5):413-423. doi: 10.1007/s10295-020-02277-5. Epub 2020 May 4. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
172353	pCMU-4	CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA
217648	pCM4.4	an ermE*p-cas9-driven expression, as an alternative plasmid