Accelerated RNA detection using tandem CRISPR nucleases.
Liu TY, Knott GJ, Smock DCJ, Desmarais JJ, Son S, Bhuiya A, Jakhanwal S, Prywes N, Agrawal S, Diaz de Leon Derby M, Switz NA, Armstrong M, Harris AR, Charles EJ, Thornton BW, Fozouni P, Shu J, Stephens SI, Kumar GR, Zhao C, Mok A, Iavarone AT, Escajeda AM, McIntosh R, Kim S, Dugan EJ, Pollard KS, Tan MX, Ott M, Fletcher DA, Lareau LF, Hsu PD, Savage DF, Doudna JA
Nat Chem Biol. 2021 Sep;17(9):982-988. doi: 10.1038/s41589-021-00842-2. Epub 2021 Aug 5. PubMed Article
Liu TY, Knott GJ, Smock DCJ, Desmarais JJ, Son S, Bhuiya A, Jakhanwal S, Prywes N, Agrawal S, Diaz de Leon Derby M, Switz NA, Armstrong M, Harris AR, Charles EJ, Thornton BW, Fozouni P, Shu J, Stephens SI, Kumar GR, Zhao C, Mok A, Iavarone AT, Escajeda AM, McIntosh R, Kim S, Dugan EJ, Pollard KS, Tan MX, Ott M, Fletcher DA, Lareau LF, Hsu PD, Savage DF, Doudna JA
Nat Chem Biol. 2021 Sep;17(9):982-988. doi: 10.1038/s41589-021-00842-2. Epub 2021 Aug 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172486 | pET_His6-SUMO-TEV-TtCsm6 | Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site. |
| 172487 | pET28a_His-TEV-EiCsm6 | Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site. |
| 172488 | pGJK_His-SUMO-LbuCas13a | Bacterial expression of codon-optimized His-SUMO-LbuCas13a. |