pET_His6-SUMO-TEV-TtCsm6
(Plasmid #172486)

• Purpose: Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.
• Depositing Lab: Jennifer Doudna
• Publication: Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. doi: 10.1038/s41589-021-00842-2. Epub 2021 Aug 5.

Backbone

• Vector backbone: pET
• Total vector size (bp): 7060
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: T. thermophilus Csm6 (codon optimized)
• Alt name: TTHB152
• Species: Synthetic
• Entrez Gene: csm6 (a.k.a. TTH_RS10780, TTHB152)
• Promoter: T7 promoter and lac operator
• Tag / Fusion Protein:
  • His-SUMO-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: SUMO forward (5'-ttattgaggctcacagagaac-3')
• 3′ sequencing primer: T7 reverse (5'-GCTAGTTATTGCTCAGCGG-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GenScript and QB3 Macrolab (UC Berkeley)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Codon-optimized TtCsm6 gene ordered as a synthetic gene from GenScript in pUC57. It was then subcloned into a bacterial expression vector by QB3 Macrolab at UC Berkeley.

Please visit https://www.medrxiv.org/content/10.1101/2021.03.19.21253328v1 for medRxiv preprint.