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Precise genomic deletions using paired prime editing.
Choi J, Chen W, Suiter CC, Lee C, Chardon FM, Yang W, Leith A, Daza RM, Martin B, Shendure J
Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01025-z. doi: 10.1038/s41587-021-01025-z.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
172655PB-HD118Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene
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172656PB-HD252Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene
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172657pU6nH1-HD10kbExpressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene
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172658Lentiguide-Blast-eNMU710Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus
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