A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9.

Collias D, Leenay RT, Slotkowski RA, Zuo Z, Collins SP, McGirr BA, Liu J, Beisel CL
Sci Adv. 2020 Jul 15;6(29):eabb4054. doi: 10.1126/sciadv.abb4054. eCollection 2020 Jul. PubMed Article

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