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Interrogation of the dynamic properties of higher-order heterochromatin using CRISPR-dCas9.

Gao Y, Han M, Shang S, Wang H, Qi LS
Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 10.1016/j.molcel.2021.07.034. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174113pHR: pPGK PYL1-sfGFP-HP1Adel72Expression of PYL1-sfGFP-HP1Adel72
174114pHR: pPGK PYL1-sfGFP-HP1AI165EExpression of PYL1-sfGFP-HP1AI165E
174115pHR: pPGK mCherry-HP1AExpression of mCherry-HP1A
174116pHR: pPGK PYL1-sfGFP-NLSExpression of PYL1-sfGFP-NLS
174118pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-PuroExpression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)
174119pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-PuroExpression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)
174132pHR: pPGK PYL1-sfGFP-HP1AExpression of PYL1-sfGFP-HP1A

Antibodies from Article