The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3.
Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, Brennan RG, Ko DC
Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174391 | pcDNA3.1-Flag-stm2585 | S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag |
| 174392 | pcDNA3.1-Flag-stm2585_Y167F | S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ |
| 174393 | pcDNA3.1-Flag-stm2585-gp130 | Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence. |
| 174394 | pcDNA3.1-Flag-gp130dimer | Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006). |
| 174395 | pcDNA3.1-Flag-gp130dimer-stm2585 | Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179) |
| 174397 | pEGFP-Flag-stm2585 | Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags. |
| 174398 | pEGFP-Flag-stm2585_Y167F | Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQ |
| 174399 | pEGFP-Flag-stm2585_S130A | Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAP |
| 174400 | pEGFP-Flag-stm2585_L139* | S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130 |
| 174401 | pWSK129-stm2585 | S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression. |
| 174402 | pWSK129-stm2585_Y167F | Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ |
| 174403 | pWSK129-stm2585_S130A | Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP. |
| 174404 | pWSK129-stm2585-gp130 | Bacterial expression of sarA-gp130 chimera under native promoter. |