A bacterial three-hybrid assay for forward and reverse genetic analysis of RNA-protein interactions.
Stockert OM, Gravel CM, Berry KE
Nat Protoc. 2022 Apr;17(4):941-961. doi: 10.1038/s41596-021-00657-4. Epub 2022 Feb 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
174787 | pRM16 | Constitutive expression of the λCI protein; used as three-hybrid control corresponding to pCW17 (addgene #174664) |