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Deletion and replacement of long genomic sequences using prime editing.
Jiang T, Zhang XO, Weng Z, Xue W
Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01026-y. doi: 10.1038/s41587-021-01026-y.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174851PE-Cas9Express PE-Cas9 in mammalian cells
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174852HEK3_pegF_18bpExpress forward pegRNA mediating 991-bp deletion and 18-bp insertion
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174853HEK3_pegR_18bpExpress reverse pegRNA mediating 991-bp deletion and 18-bp insertion
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174854HEK3_pegF_44bpExpress forward pegRNA mediating 991-bp deletion and 44-bp insertion
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174855HEK3_pegR_44bpExpress forward pegRNA mediating 991-bp deletion and 44-bp insertion
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174856HEK3_pegF_60bpExpress forward pegRNA mediating 991-bp deletion and 60-bp insertion
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174857HEK3_pegR_60bpExpress reverse pegRNA mediating 991-bp deletion and 60-bp insertion
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