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Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D.
Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W
Nat Methods. 2021 Nov 25. pii: 10.1038/s41592-021-01319-9. doi: 10.1038/s41592-021-01319-9.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
175273pAAV-DIO-NS1AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D
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175274pAAV-rtTAAAV vector mediating neuron-specific expression of rtTA
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175275pAAV-SynaptoTAG2AAV vector for mapping synaptic projections of infected neurons; expresses tdTomato to reveal neuronal soma and axons, and expresses EGFP fused to Synaptobrevin-2 to track synaptic terminals.
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175276AAV-Syn-NS1AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
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175277AAV-TRE-C-prM-E-NS1AAV vector mediating inducible expression of 4 genes (the C-prM-E-NS1) of YFV-17D
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175278AAV-TRE-NS1-dTomatoAAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
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175279AAV-TRE-NS1NFAAV vector mediating inducible expression of the NS1 gene of YFV-17D
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175280AAV-tTAAAV vector mediating neuron-specific expression of tTA
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175281FUW-C-prM-E-NS1lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.
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179952YFVdelNS1-CrepCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre
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179953YFVdelCME-mVenuspCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus
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Antibodies from Article