Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells.
Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker E
Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
80453pX330-Flag-wtSpCas9-H840ASpCas9 mammalian expression vector
Loading...
177178pDAS12125_PEAR-GFPPEAR-GFP target plasmid with split EGFP and disrupted 5' splice site
Loading...
177179pDAS12124_PEAR-GFP-preeditedPEAR-GFP control plasmid with split EGFP and intact 5' splice site
Loading...
177180pDAS12069_U6-pegRNA-mCherrypegRNA cloning plasmid with mCherry marker
Loading...
177181pDAS12222_U6-pegRNA-BFPpegRNA cloning plasmid with BFP marker
Loading...
177182pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherryplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker
Loading...
177183pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherryplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker
Loading...
177184pDAS12342_PEAR-GFP_2in1PEAR-GFP plasmid with a pegRNA that targets its own plasmid
Loading...
177185pDAS12395_PEAR-GFP_2.0-preeditedPEAR-GFP3 control plasmid with split EGFP and intact 5' splice site
Loading...
177186pDAS12489_PEAR-GFP_2in1_2.0PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid
Loading...

Antibodies from Article