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ER - lysosome contacts at a pre-axonal region regulate axonal lysosome availability.

Ozkan N, Koppers M, van Soest I, van Harten A, Jurriens D, Liv N, Klumperman J, Kapitein LC, Hoogenraad CC, Farias GG
Nat Commun. 2021 Jul 23;12(1):4493. doi: 10.1038/s41467-021-24713-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
239111pSplit-AP-V5-Protrudin (human ZFYVE27)Mammalian expression of human ZFYVE27 (protrudin) tagged with Split-AP-V5 on the N terminus
239112pSplit-EX-3xHA-RAB7AMammalian expression of canis RAB7A tagged with Split-EX-3xHA on the N terminus
239113pGB-V5-RAB7AMammalian expression of human RAB7A tagged with ddFP GB-V5 on the N terminus
239114pRA-3xHA-Protrudin (Human ZFYVE27)Mammalian expression of human ZFYVE27 (protrudin) tagged with RA-3xHA on the N terminus

Antibodies from Article