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CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation.

Le AH, Yelland T, Paul NR, Fort L, Nikolaou S, Ismail S, Machesky LM
J Cell Biol. 2021 Sep 6;220(9). pii: 212437. doi: 10.1083/jcb.202012114. Epub 2021 Jun 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
247175p16-GFP-CYRI-AExpresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with an internal GFP after proline 16, preserving the proposed N-terminal myristoylation site.
247375p16-mCherry-CYRI-AExpresses recombinant fluorescent CYRI-A (Fam49A) protein in mammalian cells, specifically mouse Cyri-A with internal mCherry after proline 16, preserving the proposed N-terminal myristoylation site.
248969p17-GFP-CYRI-BExpresses recombinant fluorescent CYRI-B (Fam49B) protein in mammalian cells, specifically human Cyri-B with internal GFP after proline 17, preserving the proposed N-terminal myristoylation site.
248970p17-mCherry-CYRI-BExpresses recombinant fluorescent CYRI-B (Fam49B) protein in mammalian cells, specifically human Cyri-B with internal mCherry after proline 17, preserving the proposed N-terminal myristoylation site.

Antibodies from Article