A plug-and-play platform of ratiometric bioluminescent sensors for homogeneous immunoassays.
Ni Y, Rosier BJHM, van Aalen EA, Hanckmann ETL, Biewenga L, Pistikou AM, Timmermans B, Vu C, Roos S, Arts R, Li W, de Greef TFA, van Borren MMGJ, van Kuppeveld FJM, Bosch BJ, Merkx M
Nat Commun. 2021 Jul 28;12(1):4586. doi: 10.1038/s41467-021-24874-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220125 | pET28a(+)-Gx-SB | Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx). |
| 220126 | pET28a(+)-Gx-LB | Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx). |