Video-based pooled screening yields improved far-red genetically encoded voltage indicators.
Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot V, Deisseroth K, Cohen AE
Nat Methods. 2023 Jul;20(7):1082-1094. doi: 10.1038/s41592-022-01743-5. Epub 2023 Jan 9. PubMed Article
Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot V, Deisseroth K, Cohen AE
Nat Methods. 2023 Jul;20(7):1082-1094. doi: 10.1038/s41592-022-01743-5. Epub 2023 Jan 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 178820 | TDG004_pLenti_CMVtight_Kir2.1_CFP | tet-on expression of an inward-rectifier potassium channel Kir2.1 carrying an CFP tag |
| 178821 | HT028_FCMV_CheRiff_EGFP | Expresses a blue-light activated cationic channelrhodopsin CheRiff carrying an EGFP tag |
| 178822 | HT103_FCMV_QuasAr6a_Citrine | Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6a carrying a Citrine expression tag |
| 178823 | HT110_FCMV_QuasAr6b_citrine | Expresses an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag |
| 178824 | HT111_Fsyn_FAS(Cre off)_QuasAr6a_Citrine | Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag |
| 178825 | HT114_Fsyn_FAS(Cre off)_QuasAr6b_Citrine | Neuronal expression (Cre-off) of an archaerhodopsin-derived genetically encoded voltage indicator QuasAr6b carrying a Citrine expression tag |
| 178826 | HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA | Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter |
| 178827 | HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA | Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter |
| 190878 | HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP | Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter |
| 190879 | HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP | Cre-on expression of soma-targeted QuasAr6b under an neuronal promoter |
| 208605 | HT028_FCMV_CheRiff_EGFP | Expressing CheRiff_EGFP under CMV promoter |
| 208606 | HT121_Fsyn_QuasAr6a(Q3_codon)_citrine | Expressing QuasAr6a_Citrine with an alternate codon |