Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students.

Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, Sorensen JL
J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 10.1128/jmbe.00242-22. eCollection 2023 Dec. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
179866	pUC57-fsr1	Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae
179868	pCOA-Leu-FvPPT1-fsr1	Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
179923	pCOA-Leu-FvPPT1	Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1