A cell-free transcription-translation pipeline for recreating methylation patterns boosts DNA transformation in bacteria.
Vento JM, Durmusoglu D, Li T, Patinios C, Sullivan S, Ttofali F, van Schaik J, Yu Y, Wang Y, Barquist L, Crook N, Beisel CL
Mol Cell. 2024 Jul 25;84(14):2785-2796.e4. doi: 10.1016/j.molcel.2024.06.003. Epub 2024 Jun 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180077 | pJV170 | Backbone to clone MTase genes for expression in TXTL |
| 180078 | pJV505 | MS.SenLT2II_L85Q expression construct to improve transformation in S. enterica LT2 |
| 180079 | pJV303 | M.SenLT2I expression construct to improve transformation in S. enterica LT2 |
| 180080 | pJV457 | M.BbrUI expression construct to improve transformation in B. breve UCC2003 |
| 180081 | pJV461 | M.BbrUIII expression construct to improve transformation in B. breve UCC2003 |