Antigen identification and high-throughput interaction mapping by reprogramming viral entry.
Dobson CS, Reich AN, Gaglione S, Smith BE, Kim EJ, Dong J, Ronsard L, Okonkwo V, Lingwood D, Dougan M, Dougan SK, Birnbaum ME
Nat Methods. 2022 Apr;19(4):449-460. doi: 10.1038/s41592-022-01436-z. Epub 2022 Apr 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 182229 | pMD2-VSVGmut | Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping |
| 182230 | pLeAPS-GFP-blast | Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene |
| 240242 | pHIV-IL13-CD8aStalk-PDGFR | Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain |
| 240243 | pHIV-IL13-PDGFRStalk-TM | Lentiviral transfer plasmid encoding IL-13 with PDGFR stalk and TM domain |
| 240245 | pHIV-IL13-IgG4hinge-PDGFR | Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain |
| 240246 | pHIV-IL13-GAPGAS-PDGFR | Lentiviral transfer plasmid encoding IL13 with GAPGAS linker and PDGFR TM |
| 240247 | pHIV-IL13-GAPGlySer-PDGFR | Lentiviral transfer plasmid encoding IL13 with GAP-Gly-Ser linker and PDGFR TM |