Duplex Labeling and Manipulation of Neuronal Proteins Using Sequential CRISPR/Cas9 Gene Editing.
Droogers WJ, Willems J, MacGillavry HD, de Jong APH
eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.2022. doi: 10.1523/ENEURO.0056-22.2022. PubMed Article
Droogers WJ, Willems J, MacGillavry HD, de Jong APH
eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.2022. doi: 10.1523/ENEURO.0056-22.2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183420 | pOC1 cloning template vector | CreON knock-in vector |
| 183421 | pOC2 cloning template vector | CreOFF knock-in vector |
| 183422 | pOC3 cloning template vector | CreON FlpOFF knock-in vector |
| 183423 | pOC4 cloning template vector | FlpON knock-in vector with FLAG-SpCas9 expression |
| 183424 | pOC5 cloning template vector | FlpOFF knock-in vector |
| 183425 | pFUGW NLS-Cre | Lentiviral Cre vector |
| 183426 | pOC1 Halo-Actb KI | CreON knock-in for Halo-beta-actin (amino acid position: before start codon) |
| 183427 | pOC1 Gria1-HA KI | CreON knock-in for GluA1-HA (amino acid position: stop codon) |
| 183428 | pOC1 Gria1-GFP-FRB KI | CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon) |
| 183429 | pOC2 GFP-Actb KI | CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon) |
| 183430 | pOC2 Gria1-GFP KI | CreOFF knock-in for GluA1-GFP (amino acid position: stop codon) |
| 183431 | pOC2 Tubb3 no donor | CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon) |
| 183432 | pOC2 Tubb3-GFP KI | CreOFF knock-in for beta3-tubulin-GFP (amino acid position: stop codon) |
| 183433 | pOC2 Gria1-Halo KI | CreOFF knock-in for GluA1-Halo (amino acid position: stop codon) |
| 183434 | pOC2 GFP-Mpp2 KI | CreOFF knock-in for GFP-MPP2 (amino acid position: A4) |
| 183435 | pOC2 Dlg4-Halo-FKBP KI | CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721) |
| 183436 | pOC3 smFP-HA-Cacna1e KI | CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5) |
| 183437 | pOC4 Actb-mRuby3 KI | FlpON knock-in for mRuby3-beta-actin (amino acid position: before start codon) |
| 183438 | pOC4 Kcnn2-smFP-myc KI | FlpON knock-in for SK2-smFP-myc (amino acid position: T571) |
| 183439 | pOC4 Grin1-smFP-V5 KI | FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20) |
| 183440 | pOC5 Tubb3-GFP KI | FlpOFF knock-in for beta3-tubulin GFP (amino acid position: stop codon) |
| 183441 | pOC5 smFP-HA-Cacna1e KI | FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5) |
| 183443 | pORANGE Tubb3-2xGFP KI | GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon) |
| 183444 | pORANGE Tubb3-3xGFP KI | GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon) |
| 183445 | pORANGE Tubb3-4xGFP KI | GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon) |
| 183446 | pOC2 CRISPIE-Halo-Homer1 KI | CreON knock-in for Halo-Homer1 (amino acid position: G2) |
| 183447 | pCAG ERT2-Cre-ERT2 lox | CreOFF tamoxifen-inducible Cre |
| 183448 | pFUGW NLS-FlpO | Lentiviral FlpO vector |
| 183449 | pOC2 Dlg4-Halo KI | CreOFF knock-in for PSD95-Halo (amino acid position: R721) |