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Monitoring the dynamics of exosomes through the tracking of Rab7 partners

Bare Y, Delalande F, Hirschler A, Cazevieille C, Lucansky V, Carapito C, Gaudin R, Deffieu MS
bioRxiv 2025.10.07.680694 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
166398pBS L30 Flag-Apex2-Rab6BRab6B fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166399pBS L30 Flag-Apex2-Rab8ARab8A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166400pBS L30 Flag-Apex2-Rab11ARab11A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166401pBS L30 Flag-Apex2-Rab10Rab10 fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166402pBS L30 Flag-Apex2-Rab27ARab27A fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166403pBS L30 Flag-Apex2-Rab18Rab18 fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
166404pBS L30 Flag-Apex2-Rab5CRab5C fused to APEX2 and a Flag tag in N-ter under control of a weak L30 promoter.
193969Lenti NbALFA-miRFP680 hygrolentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, hygromycin resistance
193972Lenti NbALFA-miRFP680 purolentivector coding for miRFP680 fused to a nanobody targeting the ALFA tag, puromycin resistance
193973Lenti NbALFA-mNeonGreen purolentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, puromycin resistance
193974Lenti NbALFA-mNeonGreen hygrolentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance
224892pBS L30 ALFA-Apex-Rab7ARab7A fused to APEX and an ALFA tag on N-term in a pBS L30 backbone.
226184pBS L30 EGFP-Rab7ARab7A fused to the enhanced green fluorescent protein N-ter under control of a weak L30 promoter.

Antibodies from Article