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Long-term imaging of individual mRNA molecules in living cells.
Guo Y, Lee REC
Cell Rep Methods. 2022 May 25;2(6):100226. doi: 10.1016/j.crmeth.2022.100226. eCollection 2022 Jun 20.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
185794cmv-sfGFP-GB1-scABscFv GFP compnent for SunRISER
185795cmv-mCherry-8xPP7mCherry labeled with 8xPP7
185796cmv-mCherry-10xPP7mCherry labeled with 10xPP7
185797ubc-nls-pcp-5xSunTag (SRv1)pcp-5xSunTag component for SunRISER SRv1
185798ubc-nls-pcp-10xSunTag (SRv1.1)pcp-10xSunTag component for SunRISER SRv1.1
185799ubc-nls-pcp-12xSunTag (SRv1.2)pcp-12xSunTag component for SunRISER SRv1.2
185800cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1
185801cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1
185802cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

Antibodies from Article