Efficient precise in vivo base editing in adult dystrophic mice.

Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han R
Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
187053	pLKO‐puro‐2A‐mdx4cv‐GFP	fluorescent reporter for adenine base editing of mdx4cv point mutation
187054	pLenti‐puro‐OgRNA_mdxE53	SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE
187055	pCMV_ABEmaxNG‐NGA	ABEmax-SpCas9-NGA
187056	pCMV_ABE‐NGC	ABEmax-SpCas9-NGC
187057	pCMV_ABE‐NG‐loop	ABEmax-SpCas9-NG-loop
187058	pCMV‐ABEmaxNGX	ABEmax-SpCas9-NGX
187059	pCMV‐ABEmaxNGX‐NGA	ABEmax-SpCas9-NGX‐NGA
187060	pCMV‐ABEmaxNGX‐NGC	ABEmax-SpCas9-NGX-NGC
187061	pCMV‐ABEmaxNGX‐loop	ABEmax-SpCas9-NGX-loop
187062	pCMV_miniABEmaxNG(GG)	miniABEmax(GG)-SpCas9-NG
187063	pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA	Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187064	pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA	Npu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187065	pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA	Gp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187066	pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA	Gp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
187067	pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)	Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation
187068	pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA	Gp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation
187069	pCMV_SpG‐ABE8e(V106W)	ABE8e(V106W)-SpG