Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.
Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, D'Este E, Heilemann M, Hiblot J, Johnsson K
J Am Chem Soc. 2023 Feb 8;145(5):3075-3083. doi: 10.1021/jacs.2c11969. Epub 2023 Jan 30.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
187070 | pCDNA5/FRT/TO_H2B-HaloTag7_T2A_mEGFP | Nuclear expression of HaloTag7 in mammalian cells |
187071 | pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFP | Nuclear expression of dHaloTag7 in mammalian cells |
187072 | pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP | Mitochondria surface expression of HaloTag7 in mammalian cells |
187073 | pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP | Mitochondria surface expression of dHaloTag7 in mammalian cells |
187074 | pCDNA5/FRT/TO_Vimentin-HaloTag7_T2A_mEGFP | Expression of HaloTag7 located at the intermediate filaments of mammalian cells |
187075 | pCDNA5/FRT/TO_Vimentin-dHaloTag7_T2A_mEGFP | Expression of dHaloTag7 located at the intermediate filaments of mammalian cells |
187076 | pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7 | Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments |
187077 | pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7 | Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix |
187078 | pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7 | Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes |
187079 | pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL | Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum |