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Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.

Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, D'Este E, Heilemann M, Hiblot J, Johnsson K
J Am Chem Soc. 2023 Feb 8;145(5):3075-3083. doi: 10.1021/jacs.2c11969. Epub 2023 Jan 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
187070pCDNA5/FRT/TO_H2B-HaloTag7_T2A_mEGFP Nuclear expression of HaloTag7 in mammalian cells
187071pCDNA5/FRT/TO_H2B-dHaloTag7_T2A_mEGFPNuclear expression of dHaloTag7 in mammalian cells
187072pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP Mitochondria surface expression of HaloTag7 in mammalian cells
187073pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFPMitochondria surface expression of dHaloTag7 in mammalian cells
187074pCDNA5/FRT/TO_Vimentin-HaloTag7_T2A_mEGFP Expression of HaloTag7 located at the intermediate filaments of mammalian cells
187075pCDNA5/FRT/TO_Vimentin-dHaloTag7_T2A_mEGFP Expression of dHaloTag7 located at the intermediate filaments of mammalian cells
187076pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments
187077pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix
187078pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes
187079pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDELCo-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

Antibodies from Article