Recording gene expression order in DNA by CRISPR addition of retron barcodes.
Bhattarai-Kline S, Lear SK, Fishman CB, Lopez SC, Lockshin ER, Schubert MG, Nivala J, Church GM, Shipman SL
Nature. 2022 Aug;608(7921):217-225. doi: 10.1038/s41586-022-04994-6. Epub 2022 Jul 27. PubMed Article
Bhattarai-Kline S, Lear SK, Fishman CB, Lopez SC, Lockshin ER, Schubert MG, Nivala J, Church GM, Shipman SL
Nature. 2022 Aug;608(7921):217-225. doi: 10.1038/s41586-022-04994-6. Epub 2022 Jul 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 187201 | pSLS.400 | Expresses retron Eco4 RT |
| 187202 | pSLS.402 | Expresses retron Eco1 RT |
| 187203 | pSLS.405 | Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2 |
| 187204 | pSLS.407 | Expresses retron Eco1 ncRNA v32, and Cas1 + Cas2 |
| 187205 | pSLS.408 | Expresses retron Eco1 ncRNA v35, and Cas1 + Cas2 |
| 187207 | pSLS.416 | Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2 |
| 187208 | pSLS.419 | Expresses wild-type retron Eco4 ncRNA, and Cas1 + Cas2 |
| 187209 | pSBK.009 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2 |
| 187210 | pSBK.010 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 2 [GCT-AGC]), and Cas1 + Cas2 |
| 187211 | pSBK.011 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 3 [CTG-CAG]), and Cas1 + Cas2 |
| 187212 | pSBK.012 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 4 [GTG-CAC]), and Cas1 + Cas2 |
| 187213 | pSBK.013 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2 |
| 187215 | pSBK.014 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 6 [CAG-CTG]), and Cas1 + Cas2 |
| 187216 | pSBK.015 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 7 [GAG-CTC]), and Cas1 + Cas2 |
| 187217 | pSBK.016 | Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 8 [GCT-TGC]), and Cas1 + Cas2 |
| 187218 | pSBK.079 | Expresses Eco1 RT and Cas1 + Cas2 |
| 187219 | pSBK.134 | Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pTet* and pBetI, respectively |
| 187220 | pSBK.136 | Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively |