Phase separation of the oncogenic fusion protein EWS::FLI1 is modulated by its DNA-binding domain.
Selig EE, Sohn EJ, Stoja A, Moreno-Romero AK, Akula S, Xu X, Bishop AJR, Libich DS
Proc Natl Acad Sci U S A. 2025 May 20;122(20):e2221823122. doi: 10.1073/pnas.2221823122. Epub 2025 May 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 188042 | pAG8Ha-EWS-FLI1_Type2 | Expresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag. |
| 188044 | pAG8Ha-EWS | Expresses His8-EWS with a TEV site for removal of the His8 tag. |
| 188047 | pAG8Ha-FLI1_DBD(276-399_F362A) | Expresses His8-FLI1_DBD(276-399_F362A) with a TEV site for removal of the His8 tag. |
| 188048 | pAG8Ha-FLI1_DBD(276-399_C299S_F362A_S390C) | Expresses His8-FLI1_DBD(276-399_C299S_F362A_S390C) with a TEV site for removal of the His8 tag. |
| 188049 | pAG8Ha-FLI1_DBD_R2L2 | Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag. |
| 188050 | pAG8Ha-FLI1_DBD_deltaAlpha4 | Expresses His8-FLI1_DBD_deltaAlpha4(276-361) with a TEV site for removal of the His8 tag. |
| 188051 | pAG8Ha-ERG_DBD(306-429_F392A) | Expresses His8-ERG_DBD(306-429_F392A) with a TEV site for removal of the His8 tag. |
| 188052 | pAG8Ha-ETV1_DBD(332-458) | Expresses His8-ETV1_DBD(332-458) with a TEV site for removal of the His8 tag. |
| 188053 | pAG8Ha-PU.1_DBD(168-270) | Expresses His8-PU.1_DBD(168-270) with a TEV site for removal of the His8 tag. |