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Structural basis for ATG9A recruitment to the ULK1 complex in mitophagy initiation.
Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. Epub 2023 Feb 15.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
188073pCAG-GST-ATG9 (828-839)-ATG101 (1-198)To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells
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188074pCAG-ATG13 (1-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
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188075pCAG-OSF-ATG13 (2-197)To make ATG101/ATG13 HORMA complex for expression in mammalian cells
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188076pCAG-MBP-ATG9Recombinant overexpression of human ATG9 in mammalian cells
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188077pCAG-MBP-ATG9-V839AExpresses human ATG9-V839A mutant in mammalian cells
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188078pCAG-MBP-ATG9-K838AExpresses human ATG9-K838A mutant in mammalian cells
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188079pCAG-MBP-ATG9-H837AExpresses human ATG9-H837A mutant in mammalian cells
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188080pCAG-MBP-ATG9-V836AExpresses human ATG9-V836A mutant in mammalian cells
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188081pCAG-MBP-ATG9-Q835AExpresses human ATG9-Q835A mutant in mammalian cells
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188082pCAG-MBP-ATG9-P834AExpresses human ATG9-P834A mutant in mammalian cells
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188083pCAG-MBP-ATG9-P833AExpresses human ATG9-P833A mutant in mammalian cells
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188084pCAG-MBP-ATG9-L832AExpresses human ATG9-L832A mutant in mammalian cells
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188085pCAG-MBP-ATG9-D830A/E831AExpresses human ATG9-D830A/E831A mutant in mammalian cells
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188086pCAG-MBP-ATG9 (1-830)Expresses human ATG9 C-terminal truncation mutant in mammalian cells
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188087pCAG-MBP-Foldon-ATG9 (692-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
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188088pCAG-MBP-Foldon-ATG9 (723-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
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188089pCAG-MBP-Foldon-ATG9 (801-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
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188090pCAG-MBP-Foldon-ATG9 (828-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
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188091pCAG-MBP-Foldon-ATG9 (830-839)To make ATG9 C-terminal tail trimer for expression in mammalian cells
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188092pCAG-MBP-ATG9 (692-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
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188093pCAG-MBP-ATG9 (801-839)To make monomeric ATG9 C-terminal tail for expression in mammalian cells
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188094pCAG-OSF-ATG13 (2-197)-K15DExpresses human ATG13 HORMA K15D mutant in mammalian cells
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188095pCAG-OSF-ATG13 (2-197)-F16DExpresses human ATG13 HORMA F16D mutant in mammalian cells
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188096pCAG-OSF-ATG13 (2-197)-K18DExpresses human ATG13 HORMA K18D mutant in mammalian cells
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188097pCAG-OSF-ATG13 (2-197)-F19DExpresses human ATG13 HORMA F19D mutant in mammalian cells
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188098pCAG-OSF-ATG13 (2-197)-W50DExpresses human ATG13 HORMA W50D mutant in mammalian cells
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188099pCAG-OSF-ATG13 (2-197)-E83LExpresses human ATG13 HORMA E83L mutant in mammalian cells
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188100pCAG-OSF-ATG13 (2-197)-Y115DExpresses human ATG13 HORMA Y115D mutant in mammalian cells
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188101pCAG-OSF-ATG13 (2-197)-Y118DExpresses human ATG13 HORMA Y118D mutant in mammalian cells
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188102pCAG-OSF-ATG13 (2-197)-L151DExpresses human ATG13 HORMA L151D mutant in mammalian cells
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188103pCAG-OSF-ATG13 (2-197)-Y152DExpresses human ATG13 HORMA Y152D mutant in mammalian cells
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188104pCAG-OSF-ATG13 (2-197)-R153DExpresses human ATG13 HORMA R153D mutant in mammalian cells
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188105pCAG-GST-ATG101-Y45DExpresses human ATG101 HORMA Y45D mutant in mammalian cells
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188106pCAG-GST-ATG101-W110D/F112DExpresses human ATG101 HORMA W110D/F112D mutant in mammalian cells
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Antibodies from Article