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Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants.

Zhang L, Li G, Zhang Y, Cheng Y, Roberts N, Glenn SE, DeZwaan-McCabe D, Rube HT, Manthey J, Coleman G, Vakulskas CA, Qi Y
Genome Biol. 2023 Apr 30;24(1):102. doi: 10.1186/s13059-023-02929-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
188542pYPQ230-D156RGateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing
188543pYPQ230-RRVGateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
188544pYPQ230-RVGateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
200187pYPQ230-RVQGateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing
200188pYPQ230-RRVQGateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

Antibodies from Article