Bryan Luikart Lab: Retroviral vectors for expression of AKT kinases with GFP and Cre recombinase

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
189008	pRubiG-T2A-AKT3-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF and Cre recombinase, separated by T2A motifs
189009	pRubiG-T2A-AKT3(S472A)-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF with a phosphodeficient mutation at Serine 472, and Cre recombinase, separated by T2A motifs.
189010	pRubiG-T2A-AKT3(S472D)-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF with a phosphomimetic mutation at Serine 472, and Cre recombinase, separated by T2A motifs.
189011	pRubiG-T2A-AKT1-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.
189012	pRubiG-T2A-AKT1(S473A)-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF with a phosphodeficient mutation at Serine 473, and Cre recombinase, separated by T2A motifs.
189013	pRubiG-T2A-AKT1(S473D)-T2A-Cre	Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF with a phosphomimetic mutation at Serine 473, and Cre recombinase, separated by T2A motifs.