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Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains.
Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA
PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. PubMed Article

Plasmids from Article

ID Plasmid Purpose
190076AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroRexpress human ACE2 with selectable markers iRFP670 and puromycin resistance gene
190077AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroRexpress human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
190078AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroRexpress Rhinolophus ferrumequinum ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
190079AttB_ACE2[R.alcyone+ferrum]-2A-TMPRSS2_IRES-iRFP670-P2A-PuroRexpress chimeric Rhinolophus alcyone ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene
190080AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroRexpress cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene
190081pcDNA3_SARS-CoV-Spike[BtKY72 RBD]express chimeric SARS-CoV spike with the BtKY72 receptor binding domain
190082pcDNA3_SARS-CoV-Spike[Khosta1 RBD]express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain
190083pcDNA3_SARS-CoV-Spike[Khosta2 RBD]express chimeric SARS-CoV spike with the Khosta-2 receptor binding domain
190084pcDNA3_BtKY72-Spikeexpress BtKY72 spike
190085pcDNA3_SARS-CoV-Spike[PRD0038 RBD]express chimeric SARS-CoV spike with the PRD0038 receptor binding domain
190086pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain
190087pcDNA3_SARS-CoV-Spike[WIV1 RBD]express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

Antibodies from Article