A bacterial dual positive and negative selection system for dCas9 activity.
Spisak S, O'Brien B, Ostermeier M
PLoS One. 2022 Jun 3;17(6):e0269270. doi: 10.1371/journal.pone.0269270. eCollection 2022. PubMed Article
Spisak S, O'Brien B, Ostermeier M
PLoS One. 2022 Jun 3;17(6):e0269270. doi: 10.1371/journal.pone.0269270. eCollection 2022. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190242 | pSelect-2 | Encodes lacI-targeting gRNA for positive selection of dCas9 activity. |
| 190243 | pSelect-6 | Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity. |
| 190244 | pSelect-7 | Encodes lacI- and pheA-targeting gRNAs for positive and negative selections of dCas9 activity. |
| 190245 | pSelect-9 | Encodes lacI- and aadA-targeting gRNAs for positive and negative selections of dCas9 activity. |